地區: | 上海 |
提供商: | SABio |
服務名稱: | PCR芯片 |
抗病毒反應PCR芯片可用于研究參與病毒先天免疫反應的84個關鍵基因的表達。三個不同家族的模式識別受體(TLRs,NLRS,RIG-I樣受體)啟動先天免疫,從而識別常見病原體如病毒等。這些受體識別和結合病毒DNA和RNA,激活下游的信號通路,從而誘導炎性細胞因子的表達,如α和β干擾素。這一芯片包含了TLRs信號效應體基因、NLRS基因和RIG-I樣受體基因,下游信號通路相關基因,I型干擾素信號通路相關基因,以及干擾素刺激基因(ISGs)。通過實時定量PCR的方法,研究者即能夠利用該芯片簡單可靠地同時檢測抗病毒反應相關基因的表達,研究特定病毒感染環境下,先天免疫信號網絡的相互作用。 Toll-Like Receptor Signaling: Receptors and Chaperones: CTSB, CTSL1, CTSS, TLR3, TLR7, TLR8, TLR9. Downstream Signaling: CHUK (IKKa), FOS, IKBKB, IRAK1, IRF3, IRF5, IRF7, JUN, MAP2K1 (MEK1), MAP2K3 (MEK3), MAP3K7 (TAK1), MAPK1 (ERK2), MAPK14 (p38 MAPK), MAPK3 (ERK1), MAPK8 (JNK1), MYD88, NFKB1, NFKBIA (I?Ba/MAD3), RELA, RIPK1, SPP1, TBK1, TICAM1 (TRIF), TNF, TRAF3, TRAF6. Responsive Genes: CCL3 (MIP-1A), CCL5 (RANTES), CD40 (TNFRSF5), CD80, CD86, CXCL10 (INP10), CXCL11 (I-TAC/IP-9), CXCL9 (MIG), IFNA1, IFNA2, IFNB1, IL12A, IL12B, IL15, IL1B, IL6. Nod-Like Receptor Signaling: Receptors and Signaling: AIM2, CARD9, CASP1 (ICE), HSP90AA1, MEFV, NLRP3, NOD2, OAS2, PSTPIP1, PYCARD (TMS1/ASC), PYDC1 (POP1), SUGT1. Responsive Genes: IL1B, IL18. RIG-I-Like Receptor Signaling: Receptors and Chaperones: CYLD, DAK, DDX58 (RIG-I), DHX58 (LGP2), IFIH1 (MDA5), ISG15 (G1P2), TRIM25. Downstream Signaling: ATG5, AZI2, CASP10 (MCH4), CASP8 (FLICE), CHUK (IKKa), DDX3X, FADD, IKBKB, IRF3, IRF7, MAP3K1 (MEKK1), MAP3K7 (TAK1), MAPK14 (p38 MAPK), MAPK8 (JNK1), MAVS, NFKB1, NFKBIA, PIN1, RELA, RIPK1, TBK1, TNF, TRADD, TRAF3, TRAF6. Responsive Genes: CXCL10 (INP10), IFNA1, IFNA2, IFNB1, IL12A, IL12B, IL8. Type-I-Interferon Signaling and Response: Signaling Pathway: IFNA1, IFNA2, IFNAR1, IFNB1, STAT1. Interferon-Stimulated Genes (ISGs): APOBEC3G, IL15, ISG15 (G1P2), MX1, TLR3. |