乳腺癌PCR芯片可用于研究與乳腺癌失調(diào)、乳腺癌細胞信號轉(zhuǎn)導(dǎo)，及其他生物過程失調(diào)有關(guān)的84個關(guān)鍵基因的表達。乳腺癌是一種異質(zhì)性疾病，在分子層面上可以分為正常乳腺癌樣、管腔、HER2像，和基底樣（稱為三陰性）腫瘤。對致癌機制的深入研究表明，某些基因表達水平的變化將影響細胞信號轉(zhuǎn)導(dǎo)和整個生物通路功能，從而導(dǎo)致癌癥發(fā)生。通過研究這些失調(diào)的基因和已知腫瘤分類標(biāo)志物即可識別乳腺癌的發(fā)生、發(fā)展和轉(zhuǎn)移。這一芯片包含了腫瘤分類的標(biāo)志物基因，腫瘤信號轉(zhuǎn)導(dǎo)相關(guān)基因，以及其他相關(guān)途徑基因，如血管生成、粘附、蛋白水解、細胞周期和細胞凋亡。通過實時定量PCR的方法，研究者即能夠利用該芯片簡單可靠地同時檢測乳腺癌發(fā)生相關(guān)基因的表達。
Tumor Classification Markers: Luminal A-C: ESR1 (ERa), FOXA1, GATA3, KRT8, KRT18, SLC39A6, TFF3, XBP1. HER2-like: ERBB2 (HER2), GRB7. Basal-like / Triple Negative: BIRC5, EGFR, KRT5, NOTCH1. Metastasis to Lung: ID1, MMP2 (Gelatinase A), PTGS2 (COX2). Signal Transduction: Steroid Receptor-Mediated: AR, BRCA1, CCNE1, CTNNB1, ESR1 (ERa), ESR2 (ER?), IGF1, KRT19, PGR, RB1. Hedgehog: BCL2, CCND1, GLI1, SNAI2. Glucocorticoid: IGFBP3, NME1 (NM23A), NR3C1 (GRL). Classical WNT: APC, CCND1, CTNNB1, SFRP1. PI3K/AKT: AKT1, ERBB2 (HER2), IGF1, IGF1R, PTEN. NOTCH: BIRC5, NOTCH1. MAPK: MAPK1 (ERK2), MAPK3 (ERK1), MAPK8 (JNK1), TP73. Epithelial to Mesenchymal Transition: CTNNB1, NOTCH1, SRC, TGFB1, TWIST1. Angiogenesis: CDH13, CTNNB1, EGF, ERBB2 (HER2), ID1, IL6, JUN, NOTCH1, PLAU (uPA), PTEN, SERPINE1 (PAI-1), SLIT2, THBS1, VEGFA. Adhesion: ADAM23, APC, BCL2, CDH1 (E-Cadherin), CDH13, CDKN2A (p16INK4), CSF1 (MCSF), CTNNB1, EGFR, ERBB2 (HER2), PTEN, TGFB1, THBS1. Proteolysis: ADAM23, CST6, CTSD, MMP2 (Gelatinase A), MMP9 (Gelatinase B), PLAU (uPA), PYCARD (ASC). Apoptosis: AKT1, APC, BAD, BCL2, CDH1 (E-CADHERIN), CDH1 (E-CADHERIN)3, CDKN1A (P21CIP1/WAF1), CDKN2A (p16INK4), GSTP1, IGF1, IL6, JUN, MUC1, NME1 (NM23A), RARB, SFN (14-3-3s), SFRP1, TP53, TP73, TWIST1. Cell Cycle: APC, BCL2, CCNA1, CCND1, CCND2, CCNE1, CDK2, CDKN1A (p21CIP1/WAF1), CDKN1C (p57KIP2), CDKN2A (p16INK4), JUN, MKI67, MYC, PTEN, RASSF1, RB1, SFN (14-3-3s), TP53. DNA Damage: APC, ATM, BRCA1, BRCA2, CCND1, CDKN1A (p21CIP1/WAF1), MAPK1 (ERK2), MGMT (AGT), MLH1, SFN (14-3-3s), TP53, TP73. Xenobiotic Transport: ABCB1 (MDR-1), ABCG2 (BCRP). Transcription Factors: AR, CTNNB1, ESR1 (ERa), ESR2 (ER?), FOXA1, GATA3, HIC1, JUN, MYC, NOTCH1, NR3C1 (GRL), PGR, PRDM2 (RIZ1), RARB, RB1, TP53, TP73, XBP1. |
