| Human Cancer Stem Cells PCR Array |
|
| The Human Cancer Stem Cells RT2 Profiler PCR array profiles the expression of 84 genes linked to cancer stem cells (CSCs). Cancer researchers have struggled with the vexing problem that although many cancer drugs dramatically reduce the size of the tumors, most cancers eventually relapse. Dynamic changes in cancer cell populations during treatment suggest that a small population of cells resistant to current therapies is ultimately responsible for the re-growth of tumors. Furthermore, studies imply that these cells may provide a reservoir for the generation and propagation of mutant cells providing further resistance to therapy. The cancer-stem-cell hypothesis posits that only a very rare population of cells within tumors has the capacity for limitless self-renewal. This concept has important therapeutic implications, and may explain why many cancers return even after treatment removes any detectable tumor cells. If current treatments do not eliminate cancer stem cells, then they may regenerate the tumor once treatment stops. Recently, advances in technology have allowed the prospective identification and purification of CSCs from various different types of cancers for further characterization. The genes profiled with this array include CSC molecular markers and genes regulating CSC proliferation, self-renewal, and pluripotency to help ensure the stability of CSC isolates in culture. Also included are genes involved in CSC asymmetric cell division, migration and metastasis, and relevant signal transduction pathways to help facilitate CSC characterization as well as the targets of therapeutics currently being tested. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes related to cancer stem cells with this array.
The RT2 Profiler PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
96-well Plate, 384-well (4 × 96) Plate, and 100-well Disc formats are available. |
|
|
|
|
Cancer Stem Cell Markers: ABCB5, ALCAM, ALDH1A1, ATXN1, BMI1, CD24, CD34, CD38, CD44, ENG, ETFA, FLOT2, GATA3, ITGA2, ITGA4, ITGA6, ITGB1, KIT, MS4A1, MUC1, PECAM1, PROM1, PTPRC, THY1.
Proliferation: EGF, ERBB2, KITLG, LIN28B, NOS2.
Self-Renewal: BMP7, DNMT1, FGFR2.
Pluripotency: KLF4, LIN28A, MYC, NANOG, POU5F1, SOX2.
Asymmetric Division: FOXP1, HDAC1, MYCN, SIRT1, WNT1.
Migration & Metastasis: AXL, ID1, IL8, KLF17, PLAT, PLAUR, SNAi1, TWIST1, TWIST2, ZEB1, ZEB2.
Loss of Stemness: ALDH1A1, CD34, DACH1, FOXA2, PECAM1, PTCH1.
Signal Transduction Pathways:
Hippo Signaling: LATS1, MERTK, SAV1, TAZ, WWC1, YAP1.
Hedgehog Signaling: PTCH1, SMO.
Notch Signaling: DLL1, DLL4, JAG1, MAML1, NOTCH1, NOTCH2.
WNT Signaling: DKK1, EPCAM, FZD7, WNT1.
PI3K/AKT/mTOR signaling: ABCG2, GSK3B.
STAT/NFκB Signaling: IKBKB, JAK2, NFKB1.
Therapeutic Targets: ABCG2, ATM, AXL, CHEK1, DDR1, DKK1, EPCAM, FZD7, GSK3B, ID1, IKBKB, JAK2, KLF17, NFKB1, PTCH1, SMO, STAT3, TGFBR1, WEE1. |
