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細胞譜系鑒定ChIP PCR 芯片 Cell Lineage Identification ChIP PCR Array

科技服務 > PCR芯片實驗服務 > 細胞譜系鑒定ChIP PCR 芯片 Cell Lineage Identification ChIP PCR Array

細胞譜系鑒定ChIP PCR 芯片 Cell Lineage Identification ChIP PCR Array

Cell Lineage Identification ChIP PCR Array
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地區：上海
簡介：Cell Lineage Identification ChIP PCR Array
提供商：SAB
服務名稱：Cell Lineage Identification ChIP PCR Array
Human Cell Lineage Identification ChIP PCR Array

The Human Cell Lineage EpiTect Chip qPCR Array profiles the histone modification status or “histone code” of 84 genes key to cellular differentiation. Histone modifications define chromatin structure, regulate gene activity and specify cellular status. During embryonic development, pluripotent stem cells differentiate into three germ layers: ectoderm, mesoderm and endoderm. These germ layers eventually differentiate into multipotent stem cells (progenitors), which progress into terminally differentiated cells. These developmental processes require tightly regulated and carefully timed gene expression changes. Epigenetic histone modifications are one such mechanism of regulation, where gene promoters are activated, repressed, or "poised" in an intermediate state. This array contains gene markers for specific cell types throughout cellular lineage progression, including pluripotent stem cells, progenitor cells from each of the three germ layers, and terminally differentiated cells. Surveying the histone modification pattern of these promoters can help explain the observed up- or down-regulation of their expression, thereby elucidating differentiation mechanisms, and can help validate the correct epigenetic status of differentiation programs and terminally differentiated cells. Using chromatin immunoprecipitation and this real-time PCR Array, you can easily and reliably analyze the histone modification patterns associated with a focused gene panel of cellular lineage progression markers.
The EpiTect Chip qPCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.



Pluripotent Markers: DNMT3B, GDF3 (VGR-2), LEFTY1, NANOG, PODXL, POU5F1 (OCT4), ZFP42. 
Germ Layers: 
Ectoderm: FGF5, FOXD3, OTX2, ZIC1. 
Neuroectoderm: GBX2, NEUROG2. 
Mesoderm: BMP4, CD34, DCN, GATA2, HAND1, IGF2, MIXL1, PDGFRA, RUNX1 (AML1), T (Brachyury). 
Endoderm: FOXA1, GATA1, GATA6, HNF4A, SOX17, SOX7. 
Progenitors: 
Ectoderm: 
Neuronal: FABP7, HES5, PROM1, SOX2. 
Immature Neuron: DCX. 
Immature GABA Neuron: GAD2, SLC32A1. 
Limbal Progenitor: ENO1, MSLN. 
Motor Neuron Progenitor: FOXG1, OLIG2. 
Oligodendrocyte Progenitor: NKX2-2, OLIG2. 
Mesoderm: 
Early Cardiomyocyte: HAND2. 
Early B Cell: CD79A. 
Early T Cell: CD3E, PTCRA. 
Muscle Stem Cell: CD34. 
Endoderm: 
Pancreatic Islet Cell: KRT19. 
Hepatic Stem Cell: APOH, DPP4, MAP3K12. 
Terminal Differentiation: 
Ectoderm: 
Keratinocyte: KRT10, KRT14. 
Melanocyte: TYR. 
Mature Neuron: NEUROD1. 
Cholinergic Neuron: CHAT. 
GABA Neuron: GAD1. 
Glutamatergic Neuron: SLC17A6, SLC17A7. 
Astrocyte: GALC, GFAP. 
Ganglion Cell: POU4F2. 
Photoreceptor Cell: RCVRN. 
Mesoderm: 
Skeletal Muscle Cell: MYH1. 
Smooth Muscle Cell: MYH11, SMTN. 
Cardiomyocyte: MYL3, MYH7, NPPA, RYR2. 
Osteoblast: IBSP. 
Osteoclast: CTSK. 
Chondrocyte: COL10A1, COMP. 
Macrophage: CCR5. 
Endoderm 
Hepatocyte: ALB, G6PC, TAT. 
Cholangiocyte: ITGB4. 
Beta Cell: INS, SLC2A2. 
Exocrine Cell: CPA1. 
Lung Cell: SFTPB, SFTPD. 
Proximal Tubule Cell: AQP1, MIOX.


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