骨質(zhì)疏松癥PCR基因芯片可用于研究骨質(zhì)疏松癥(OP)的發(fā)病所涉及的84個(gè)基因的表達(dá)。高齡、性別、缺乏運(yùn)動(dòng)是OP發(fā)生的主要風(fēng)險(xiǎn)因素,其他因素還包括絕經(jīng)后婦女類(lèi)固醇激素減少等。OP的骨重建過(guò)程通常是一種病態(tài)的失衡并伴隨著RANKL/OPG信號(hào)的失衡,其中RANKL水平的升高有利于再吸收,通過(guò)破骨細(xì)胞的形成、功能、以存活,降低低骨礦物質(zhì)密度(BMD)的影響。大量的研究工作正在進(jìn)行,以確定OP發(fā)病的分子機(jī)制,為治療、預(yù)防及早期診斷獲得新的標(biāo)志物。該芯片涉及的基因包含:Wnt和BMP信號(hào)轉(zhuǎn)導(dǎo)通路,細(xì)胞外基質(zhì),與骨基質(zhì)重塑相關(guān)的細(xì)胞因子和生長(zhǎng)因子,與成骨細(xì)胞和破骨細(xì)胞活性有關(guān)的基因等。利用實(shí)時(shí)定量PCR,研究者可以方便并且可信地分析在骨質(zhì)疏松癥有關(guān)的基因的表達(dá)。 Bone Remodeling & BMP Signaling: ADCY10, ALOX12, ALOX15, ALOX5, ALPL, BGLAP, BMP2, CLCN7, COL1A1, COL1A2, COMT, CRTAP, CTSK, ENPP1, HSD11B1, IGF1, ITGA1, ITGB3, MMP2, MTHFR, NFATC1, NOG, NOS3, P2RX7, PLOD2, RUNX2, SFRP1, SOST, SPARC, SPP1, STAT1, TIMP2, TWIST1, WNT10B, WNT3A. Calciotropic Hormones & Receptors: AR, CALCA, CALCR, CASR, CYP17A1, CYP19A1, DBP, ESR1, ESR2, ESRRA, NR3C1, PRL, PTH, PTH1R, PTHLH, SHBG, TSHR, VDR. Cytokines, Growth Factors & Receptors: BMP2, BMP7, CD40, CNR2, FGFR1, FGFR2, GHRH, IGFBP2, IL15, IL6, IL6R, LEPRE1, LRP1, LRP5, LRP6, LTA, LTBP2, MSTN, NPY, TGFB1, TNFAIP3, TNFRSF11A (RANK), TNFRSF11B (OPG), TNFRSF1B, TNFSF11 (RANKL), VEGFA. Osteoblast Activity & Differentiation: ALPL, BGLAP, BMP2, RUNX2. RANK/RANKL/OPG Signaling: TNFRSF11A (RANK), TNFRSF11B (OPG), TNFSF11 (RANKL). WNT/β-catenin Pathway: DKK1, LRP1, LRP5, LRP6, SFRP4, SOST, TWIST1, WNT10B, WNT3A. Other Genes: ACP5, CA2, LEP, MAB21L2. |