| Human Cancer Stem Cells miRNA PCR Array: MIHS-118Z |
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The Human Cancer Stem Cells miScript miRNA PCR Array profiles the expression of 84 miRNAs involved in cancer stem cell (CSC) biology. Cancer researchers have struggled with the vexing problem that although many cancer drugs dramatically reduce the size of the tumors, most cancers eventually relapse. Dynamic changes in cancer cell populations during treatment suggest that a small population of cells resistant to current therapies is ultimately responsible for the re-growth of tumors. Furthermore, studies imply that these cells may provide a reservoir for the generation and propagation of mutant cells, providing further resistance to therapy. The cancer stem cell hypothesis posits that only a very rare population of cells within tumors has the capacity for limitless self-renewal. This concept has important therapeutic implications, and may explain why many cancers return even after treatment removes any detectable tumor cells. If current treatments do not eliminate CSCs, then they may regenerate the tumor once treatment stops. Advances in technology have allowed the prospective identification and purification of CSCs from various different types of cancers for further characterization. Recent studies support the critical regulatory roles of the miRNAs represented by this array in CSC pluripotency, self-renewal, differentiation, metastasis, and tumor suppression. Profiling the differential expression of miRNAs in CSCs would allow a better understanding of miRNA function in CSCs and may provide invaluable insights into the molecular mechanisms of tumorigenesis and tumor progression. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of cancer stem cell miRNAs can be easily and reliably analyzed with this miScript miRNA PCR Array.
96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available. |
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Pluripotency & Differentiation: let-7a-5p, miR-122-5p, miR-122-5p, miR-134-5p, miR-137, miR-145-5p, miR-181a-5p, miR-181c-5p, miR-183-5p, miR-184, miR-200a-3p, miR-200c-3p, miR-320d, miR-34a-5p.
Self-Renewal & Proliferation: miR-134-5p, miR-137, miR-141-3p, miR-182-5p, miR-183-5p, miR-184, miR-200b-3p, miR-203a, miR-21-5p, miR-296-5p, miR-302a-3p, miR-429, miR-9-5p, miR-96-5p.
Migration & Metastasis: let-7a-5p, let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, let-7e-5p, let-7f-5p, let-7g-5p, let-7i-5p, miR-16-5p, miR-17-5p, miR-181b-5p, miR-200a-3p, miR-200b-3p, miR-200c-3p, miR-22-3p, miR-302a-3p, miR-373-3p, miR-517a-3p, miR-9-5p.
Oncogenesis & Tumor Suppression: let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, let-7e-5p, let-7f-5p, let-7g-5p, let-7i-5p, miR-181b-5p, miR-373-3p, miR-517a-3p.
Differentially Expressed miRNAs:
Up-Regulated: miR-105-5p, miR-106b-5p, miR-125b-5p, miR-132-3p, miR-135b-5p, miR-142-3p, miR-146a-5p, miR-146b-5p, miR-150-5p, miR-155-5p, miR-16-2-3p, miR-193a-3p, miR-199b-5p, miR-20a-5p, miR-221-3p, miR-222-3p, miR-223-3p, miR-25-3p, miR-299-5p, miR-29b-3p, miR-31-5p, miR-409-3p, miR-423-5p, miR-455-3p, miR-455-5p, miR-494-3p, miR-516a-5p, miR-522-3p, miR-744-5p.
Down-Regulated: miR-103a-3p, miR-10a-5p, miR-1181, miR-1207-5p, miR-128-3p, miR-130a-3p, miR-15a-5p, miR-15b-5p, miR-185-5p, miR-425-5p, miR-451a, miR-486-5p, miR-548d-5p, miR-636.
Regulated: miR-107, miR-142-5p, miR-151a-3p, miR-365a-3p.
